國家獸藥產業(yè)技術創(chuàng)新聯(lián)盟 National veterinary drug industry technology innovation alliance |
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化學發(fā)光免疫分析(chemiluminesecence immunoassay,CLIA)是用于疫病檢測的一種免疫檢測技術,是基于免疫反應原理,結合化學發(fā)光檢測技術而建立的。與放射免疫分析、酶免疫分析等標記免疫技術相比,具有無放射性污染、靈敏度高和特異性強的特點,已被廣泛應用于疫病流行病學調查、診斷、藥物分析以及微生物檢測等方面。本文簡要介紹了CLIA技術的基本原理及發(fā)展,從細菌、病毒和寄生蟲檢測方面,綜述了近年來該技術在動物疫病檢測中的應用,提出該技術正向高特異性、高靈敏度、高通量、快速和自動化檢測的方向發(fā)展,這為今后CLIA在動物疫病檢測中的應用研究提供參考。
Application of Chemiluminescence Immunoassay in Detection of Animal Diseases
As a kind of immunological technology for detection of animal diseases,chemiluminescence immunoassay(CLIA)is developed in combination with chemiluminescence detection technology based on the principle of immune response,which is characterized by high sensitivity and good specificity with no radioactive pollution compared to the immunolabelling technology such as radioimmunoassay(RIA)and enzyme immunoassay(EIA),and has been widely used for epidemiological investigation and diagnosis of animal diseases as well as drug analysis and microbial detection. In this paper,the basic principle and development of CLIA technology were introduced,and its application in animal disease detection in recent years was summarized from the aspects of bacteria,virus and parasite detection. It was shown that chemiluminescence immunoassay was turning to be a rapid and automatic detection technology with high specificity,high sensitivity and high throughput. This study would provide some reference for application of CLIA in animal disease detection in the future.
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國家獸藥產業(yè)技術創(chuàng)新聯(lián)盟 National veterinary drug industry technology innovation alliance |
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