科研動(dòng)態(tài)

牛結(jié)節(jié)性皮膚?。╨umpy skin disease，LSD）是由牛結(jié)節(jié)性皮膚病病毒（lumpy skin disease virus，LSDV）引起的急性、亞急性傳染病。我國(guó)于2019 年8 月在新疆伊犁地區(qū)首次確診該病。快速準(zhǔn)確檢測(cè)是防控該病的關(guān)鍵。目前，應(yīng)用常規(guī)PCR、實(shí)時(shí)熒光定量PCR、高分辨率熔解曲線分析、環(huán)介導(dǎo)等溫?cái)U(kuò)增等分子檢測(cè)方法，已實(shí)現(xiàn)了LSDV與其同屬不同種病毒的通用檢測(cè)和鑒別檢測(cè)以及LSDV野毒株與疫苗株的區(qū)分；應(yīng)用病毒中和試驗(yàn)、酶聯(lián)免疫吸附（ELISA）和蛋白印跡試驗(yàn)（WB）等免疫學(xué)方法，可進(jìn)行羊痘病毒屬的特異性抗原與抗體檢測(cè)，而對(duì)于LSDV特異性抗體檢測(cè)方法尚有待研究。LSD作為一種新傳入我國(guó)的外來(lái)動(dòng)物疫病，有必要運(yùn)用快速準(zhǔn)確的分子或免疫學(xué)檢測(cè)方法加強(qiáng)監(jiān)測(cè)。本文概述了LSD診斷方法研究進(jìn)展，為我國(guó)LSD診斷技術(shù)研發(fā)提供參考。

Research Progress on Diagnostic Methods of Lumpy Skin Disease

Lumpy skin disease（LSD），an acute and subacute infectious disease，is caused by lumpy skin disease virus（LSDV）. In August 2019，Chinese first case was confirmed in Yili Prefecture，Xinjiang. To prevent and control the disease is subject to rapid and accurate detection. Presently，the goal of generic detection of LSDV and its congeneric virus of different species as well as differential diagnosis of wild strains and vaccine strains have been achieved by a series of molecular detection methods，such as conventional PCR，real-time fluorescent quantitative PCR，high-resolution melting（HRM）curve analysis，loop mediated isothermal amplification（LAMP）；the specific antigen and antibody of capripoxvirus could be detected by the immunological methods，such as virus neutralization test（VNT），enzyme-linked immunosorbent assay（ELISA），Western Blot（WB），etc. However，the detection methods for specific antibodies against LSDV still need further studies. As a new exotic animal disease，LSD should be further monitored by rapid and accurate molecular or immunological methods. In conclusion，relevant research progress was summarized for the purpose of providing some references for the development of LSD diagnosis technology in China.

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