科研動(dòng)態(tài)

為簡(jiǎn)化動(dòng)物血液樣品中藍(lán)舌病病毒（BTV）檢測(cè)及研究方法，采用2種酶聯(lián)免疫吸附試驗(yàn)（ELISA），對(duì)采自云南省德宏州芒市一家肉牛養(yǎng)殖場(chǎng)的30對(duì)牛同源血漿及血清樣品進(jìn)行BTV總抗體及VP7抗體檢測(cè)。BTV總抗體ELISA檢測(cè)結(jié)果顯示，同源血漿與血清之間的陽(yáng)/陰性結(jié)果一致率為100%，ΔPI在±5%以內(nèi)的樣品占87%；VP7抗體ELISA檢測(cè)結(jié)果顯示，同源血漿與血清之間的陽(yáng)/陰性結(jié)果一致率為93%，ΔOD在±0.05范圍內(nèi)的樣品占87%。結(jié)果表明：利用ELISA檢測(cè)動(dòng)物血樣中BTV抗體時(shí)，血漿樣品可替代血清樣品；血漿樣品的溶血程度不影響檢測(cè)結(jié)果，但粗制血漿中的非溶解性雜質(zhì)可能影響檢測(cè)結(jié)果，需要使用離心除雜后的血漿樣品。

Comparison of the Test Results of Antibody against Bluetongue Virus in Homologous Plasma and Serum by ELISA

In order to simplify the test and research method of bluetongue virus（BTV）in animal blood samples，30 pairs of bovine homologous plasma and serum samples collected from a beef cattle farm in Mang City，Dehong Prefecture，Yunnan Province were tested for antibodies against BTV and VP7 protein. The results showed that，for the detection of total antibodies of BTV，the consistency rate of the positive/negative results of homologous plasma and serum was 100%，and samples with ΔPI less than ±5% accounted for 87%；as tested by VP7 Ab ELISA kit，the consistency rate of the positive/negative results of homologous plasma and serum was 93%，and samples with ΔOD less than ±5% accounted for 87%. Therefore，serum samples might be replaced by plasma samples when the antibody against BTV in animal blood was tested by ELISA，and the test results could not be affected by the hemolysis level of plasma samples rather than the insoluble impurities in crude plasma which should be removed by centrifugation before being used.

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