科研動態(tài)

為了解山東省豬流行性腹瀉病毒（porcine epidemic diarrhea virus，PEDV）基因組遺傳演化特征，將山東省濱州市某豬場的PEDV陽性樣品接種Vero E6細胞，結(jié)果成功分離到1株P(guān)EDV，將其命名為SD20BZ01株。通過RT-PCR分段擴增病毒基因組，經(jīng)測序、拼接獲得SD20BZ01株全基因組序列，并將其與GenBank收錄的參考毒株序列進行同源性比對和遺傳進化分析。全基因組序列分析結(jié)果顯示，SD20BZ01株與我國2010年以來流行的GII-b亞群變異株序列同源性為97.8%~98.7%，明顯高于CV777、DR13、SM98、SD-M等早期經(jīng)典毒株，因此SD20BZ01屬于GII-b亞群變異株?；赟基因的遺傳進化關(guān)系與基于全基因組序列的遺傳進化關(guān)系基本一致。與CV777株相比，SD20BZ01株S蛋白在中和抗原表位COE處有10個氨基酸突變，與近年來變異株S蛋白的氨基酸序列基本一致，說明GII-b亞群PEDV的變異規(guī)律逐漸趨于穩(wěn)定。本研究為山東省PEDV流行病學研究及其流行控制策略的制定提供了理論依據(jù)。

Isolation，Identification and Genetic Evolution Analysis of Porcine Epidemic Diarrhea Virus SD20BZ01 Strain in Shandong Province

In order to investigate the genetic evolution characteristics of porcine epidemic diarrhea virus（PEDV）in Shandong Province，PEDV-positive samples collected from a swine farm in Binzhou City of Shandong Province were inoculated with Vero E6 cells，then a strain of PEDV was successfully isolated，then named as SD20BZ01. Viral genome fragments were amplified by RT-PCR to obtain its whole genome sequence through sequencing and splicing，then the homology and genetic evolution relationship were compared with the reference strain registered in GenBank. It was found that，by the analysis of whole genome sequence，the homology of SD20BZ01 and GII-b variants prevalent in China since 2010 was from 97.8% to 98.7%，which was significantly higher than early classical strains like CV777，DR13，SM98 and SD-M. It was determined that SD20BZ01 was belonged to GII-b subtype variants. The genetic evolutionary relationship based on S gene was generally consistent with that based on the whole genome sequence. Compared with CV777，the S protein of SD20BZ01 harbored 10 amino acid mutations in the neutralizing antigen epitope，while was basically consistent with the amino acid sequence of S protein of variants prevalent in recent years，indicating that the rules of variation of GII-b subtype PEDV tended to be stable gradually. A theoretical basis was thus provided for future epidemiological research as well as development of prevention and control strategy against PEDV in Shandong Province.

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